Étude des facteurs associés à la supplémentation en vitamine A en routine à l'âge de six mois au centre de santé de Thiès en 2019-2020 (Sénégal) / NA

Introduction :La supplémentation en vitamine A (SVA) est une intervention à haut impact en matière de survie de l'enfant. Le Sénégal, conformément aux recommandations de l'Organisation mondiale de la santé, préconise la SVA dès six mois et tous les six mois jusqu'à cinq ans. Cependant, les données sur le délai de la SVA sont limitées. L'objectif était d'identifier les facteurs associés à la SVA en temps voulu, c'est-à- dire juste à l'âge de six mois. Méthodes : Cette étude transversale a eu lieu à Thiès, une ville située à 70 km de Dakar, capitale sénégalaise. La population d'étude était constituée de 400 enfants âgés de 06 à 11 mois et vus lors des séances de vaccination de routine au centre de santé de référence du district sanitaire de Thiès. La collecte des données a eu lieu du 02 décembre 2019 au 21 janvier 2020 à l'aide d'un entretien individuel avec les mères et du carnet de santé mère-enfant. Le délai de SVA est déterminé en faisant la différence entre la date de naissance et la date où l'enfant a reçu la capsule de vitamine A. Une régression logistique a été réalisée de sorte à identifier les facteurs qui influent sur ce délai ; estimant l'odds ratio ajusté et son intervalle de confiance à 95% (IC95%). Résultats : Les enfants sont nés de mères âgées en moyenne de 27,08±6,34 ans. La proportion de ceux ayant reçu les capsules de vitamine A était de 97,24%. En revanche, seuls 59,28% avaient bénéficié de cette supplémentation en temps voulu, c'est-à-dire juste à l'âge de six mois. Les facteurs positivement associés à la SVA en temps voulu étaient la résidence en zone périurbaine (ORa= 3,81 ; IC95%=1,63 ­ 10,1 ; p-value=0,004), le statut maternel sans emploi (ORa=1,75 ; IC95% =11,12 ­ 2,75 ; p-value=0,015), l'âge maternel supérieur ou égal à 35 ans (ORa=2,94 ; IC95%=1,41 ­ 6,48 ; p-value=0,005), le sexe féminin (ORa=1,77 ; IC95%=1,15 ­ 2,74, p-value=0,009), la vaccination par le PENTA 3 juste à l'âge de 14 semaines (ORa=13,5 ; IC95%=2,67 ­ 247, p-value=0,012). Conclusion : La couverture en SVA à l'âge approprié est faible. Beaucoup d'enfants ont bénéficié de la SVA avec un retard ; ce qui les expose à la malnutrition. Les interventions de promotion de la SVA devraient inclure la distribution des suppléments de vitamine A à l'aide des visites à domicile dans la zone urbaine de Thiès, l'aménagement du temps de travail des femmes, la sensibilisation des jeunes mères, l'intégration desservices de vaccination et de supplémentation en vitamine A. Ces efforts de promotion devraient prendre en compte la dimension genre

Clinical characteristics and risk factors of male patients with alcohol-induced psychotic disorders / 上海交通大学学报（医学版）

Objective: To explore the clinical characteristics and risk factors of male patients with psychotic disorders caused by alcohol. Methods: The clinical data of 52 male inpatients with alcohol use and related disorders treated in the Department of Addiction of Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine from May 2018 to December 2019 were collected. There were 19 cases of alcohol-induced psychotic disorder (alcohol-induced psychosis disorder, AIPD) group and 33 cases of non-AIPD group. The risk factors of AIPD were obtained by regression analysis. Results: AIPD patients had a higher incidence of hallucinations (47.4%) and delusions of murder (47.4%). Compared with non-AIPD groups, Logistic regression analysis of AIPD patients showed that vitamine B12 (VB12) serum level (P=0.011, OR=0.995) and total cholesterol serum level (P=0.039, OR=1.874) were associated with the occurrence of AIPD. Conclusion: Patients with AIPD have psychiatric symptoms mainly consisting of hallucinations and delusions of murder. When patients' laboratory tests show abnormal serum VB12 and serum total cholesterol levels, attentions should be paid to the occurrence of AIPD.

Does the addition of vitamin E to conventional UHMWPE improve the wear performance of hip acetabular cups? Micro-Raman characterization of differently processed polyethylene acetabular cups worn on a hip joint simulator

In knee replacements, vitamin E-doped ultra-high molecular weight polyethylene (UHMWPE) shows a better wear behavior than standard UHMWPE. Therefore, different sets of polyethylene (PE) acetabular cups, i.e. standard UHMWPE and cross-linked polyethylene irradiated with 50 kGy and 75 kGy, were compared, at a molecular level, with vitamin E-doped UHMWPE to evaluate their wear performance after being tested on a hip joint simulator for five million cycles. Unworn control and worn acetabular cups were analyzed by micro-Raman spectroscopy to gain insight into the effects of wear on the microstructure and phase composition of PE. Macroscopic wear was evaluated through mass loss measurements. The data showed that the samples could be divided into two groups: 1) standard and vitamin E-doped cups (mass loss of about 100 mg) and 2) the cross-linked cups (mass loss of about 30-40 mg). Micro-Raman spectroscopy disclosed different wear mechanisms in the four sets of acetabular cups, which were related to surface topography data. The vitamin E-doped samples did not show a better wear behavior than the cross-linked ones in terms of either mass loss or morphology changes. However, they showed lower variation at the morphological level (lower changes in phase composition) than the UHMWPE cups, thus confirming a certain protecting role of vitamin E against microstructural changes induced by wear testing.

Effect and Mechanism of Reactive Oxygen Species on Apoptosis of Human Hepatocellular Carcinoma Cells Induced by Fenretinide / 胃肠病学

Background:Fenretinide,which is capable of generating reactive oxygen species( ROS ),has emerged as a promising antineoplastic agent based on numerous in vitro and in vivo studies and clinical chemoprevention trials. Preliminary studies showed that fenretinide could induce apoptosis in human hepatocellular carcinoma( HCC)cells in vitro, however,the precise mechanism was not clarified. Aims:To elucidate the effect of ROS on apoptosis of human HCC cells induced by fenretinide and the underlying mechanism. Methods:Human HCC cell line Huh-7 was treated with antioxidant vitamin E,fenretinide or their combination,respectively. ROS in live cells was evaluated by confocal microscopy and flow cytometry;cell viability and apoptosis were assessed by CellTiter-Glo Luminescent Cell Viability Assay Kit and Caspase-Glo3/7 Assay Kit;expression and intracellular localization of nuclear receptor Nur77,as well as expression of stress-induced transcription factor GADD153 were measured by immunofluorescence staining and Western blotting,respectively. Results:Vitamin E pretreatment fully blocked the fenretinide-induced ROS production. In Huh-7 cells pretreated with vitamin E,cell apoptosis induced by fenretinide was significantly reduced(P<0. 05). Furthermore,effect of vitamin E pretreatment was noteworthy on reducing fenretinide-induced GADD153 expression, while no significant impact on fenretinide-induced Nur77 expression and translocation was observed. Conclusions:Elimination of ROS by vitamin E can abrogate the pro-apoptotic effect of fenretinide on Huh-7 cells,which indicates the participation of ROS in fenretinide-induced apoptosis of human HCC cells. Its mechanism might be associated with induction of GADD153 protein expression.

Enfermedad de Kawasaki en un infante / Kawasaki disease in a child

Se presenta el caso clínico de un infante de 4 años de edad, con antecedente de amigdalitis a repetición, ingresado en el Hospital Infantil Norte Docente "Dr. Juan de la Cruz Martínez Maceira" de Santiago de Cuba por presentar, desde hacía 9 días, fiebre de 38 y 39 °C, odinofagia, aumento de volumen del cuello y erupción cutánea generalizada. Teniendo en cuenta el examen físico y los resultados de los estudios complementarios efectuados se diagnosticó la enfermedad de Kawasaki, por lo cual se indicó tratamiento con Intacglobin®, Aspirina® y vitaminoterapia. El paciente evolucionó favorablemente y egresó de la institución hospitalaria sin complicaciones.

The case of a 4 year-old child with history of recurrent tonsillitis, who was admitted to "Dr. Juan de la Cruz Martínez Maceira" Northern Teaching Children Hospital of Santiago de Cuba is reported for presenting with temperature of 38 and 39°C, odynophagia, enlargement of the neck and generalized rash in the past nine days. Based on physical examination and the results of complementary tests the Kawasaki disease was diagnosed, for which a treatment with Intacglobin®, Aspirin®, and vitamin therapy was indicated. The patient made good progress and was discharged from the hospital without complications.

Protective effect of vitamin D3 on ocular structure in diabetic rat / 中华实验眼科杂志

BackgroundResearch demonstrated that vitamin D3 mediated by its receptor has the potent nonclassical effects,including immunomodulatory,antiinflammatory,and neuroprotective properties,and it can enhance the secretion and sensitivity of insulin and therefore down-regulate hyperglycemia and attenuate the corneal edema.ObjectiveThe present study was to investigate the protective effect of vitamin D3on ocular structure in experimental diabetic rat.Methods Twenty-two healthy SPF C57BL/6 rats were randomly divided into vitamine D3 group (8 rabbits),diabetic control group ( 11 rabbits) and normal control group ( 3 rabbits).2％ streptozotocin ( STZ,175 mg/kg)was intraperitoneally injected to create the diabetic models in the rats of the vitamine D3 group and diabetic control group.Blood glucose was examined for 3 times in the third day after STZ injection,and the rats with the blood glucose concentration ＞16.7 mmol/L was identified as the successful diabetic models.After modeling,the rat tail blood was collected for the monitoring of blood glucose.Two weeks after modeling,vitamine D3 was intraperitoneally injected in each week for 5 times.The fundus was examined using direct ophtalmoscope,and the eyeballs were obtained under the excessive anesthesia for the measurement of thickness of the central cornea,retina and choroids by histopathological examination once a week for 7 weeks after administration of vitamin D3.The administration of the animals complied with the Statement of ARVO.ResultsThe corneal edema appeared with the corneal thickness of (339.14± 11.13) μm in the first week and gradually attenuated with time elapse after modeling in the diabetic group ( F =382.446,P =0.000).The corneal thickness values were significantly decreased from the second week through the seventh week in the vitamin D3 group compared with diabetic control group(P＜0.05).The atrophy of the corneal epithelium was found from the fifth week to the seventh week in diabetic control group,but that in vitamin D3 group was slight (P＜0.05).The gradually thinning of the choroids was seen from the first week to the seventh week in the diabetic control group ( F =437.411,P =0.000 ),however,the thickness values in the vitamin D3 group were significantly increased in comparison with the diabetic control group in various time points (P＜0.05).The retina thickness was gradually reduced during the seven-week duration in the diabetic control group (F =91.859,P =0.000),but no significant change was identified in retina thickness in the vitamin D3 group(P＞0.05).ConclusionsVitamin D3 has prevent and therapeutic effects on experimental diabetic oculopathy.

Lycopene - tomato's secret weapon against oxidative stress.

Back ground: Lycopene, 40 carbon acyclic carotenoid containing 11 conjugated double bonds, is a phytochemical found in tomatoes and other red fruits. Oxygen derived free radicals are the most reactive species and as an antioxidant lycopene has a singlet oxygen quenching ability twice as high as that of ?-carotene and 10 times higher that of ?-tocoferol, lycopene participate in a host of chemical reactions to protect critical cellular biomolecules including lipid, proteins and DNA. Materials and Methods: The present study include 30 subjects having oxidative stress, age between 40-60 years, nonsmoker, with no history of chronic systemic illness and no medication were taken as patients.30 patients matched healthy subjects were taken as control. All subjects were selected from outpatient department of NSCB Medical College Jabalpur M.P. After estimation of base line antioxidant enzyme and vitamins, we supplement 180 gm of tomato (products like soup, paste. ketchup) contain 12 mg of lycopene to the patient group. After 60 days of lycopene supplementation oxidative stress biomarkers like SOD, GPX, GR, GSH, lipid peroxidation product MDA and other antioxidant vitamins A, vitamin C, vitamin E were estimated in patient's blood sample. Results: The main result of the study revealed that lipid per oxidation product MDA was found to be decreased significantly but after lycopene supplementation levels were improved. The results of SOD , GPX, GR, GSH,Vitamin A ,Vitamin E and Vitamin C were significantly increased after lycopene supplementation, it indicates the improved antioxidant profile after the supplementation of lycopene. Conclusion: There was a significant decrease in oxidative stress after the supplementation of lycopene therefore the study suggest that body's internal production of antioxidant is not enough to neutralize all free radicals, so increased dietary intake of antioxidant lycopene in the form of tomato products is beneficial, which is easily available in developing country like India.

The effects of famotidine combined with vitamin K1 on the therapy of neonatal alimentary tract hemorrhage / 中国基层医药

Objective To observe the therapeutic efficacy of famotidine combined with vitamin K1 on neonatal alimentary tract hemorrhage. Methods 69 neonates with neonatal alimentary tract hemorrhage were divided into control group (24 neonates who accepted the conventional therapy combined with vitamin K,) and therapy group (45 neonates who got the treatment of control group combined with famotidine). The effects were evaluated by the offtime of hemorrhage. Results The total curative effect of the therapy group was 95.6% .which significantly higher than that in control group(70. 8% ) ,P < 0.05. Conclusion Famotidine combined with vitamine K1 could improve the therapeutic efficacy of the treatment of neonatal alimentary tract hemorrhage. It was a safe and effective method in the treatment of neonatal alimentary tract hemorrhage.

Expression change of hepatic gelsolin and potential effect of Xuebijing injection in septic rats / 中华创伤杂志

Objective To investigate the expression changes of hepatic gelsolin (GSN) and the potential effect of traditional Chinese medicine Xuebijing injection in septic rats. Methods A total of 104 male Wistar rats were randomly divided into four groups, ie, normal control group (n = 8), sham operation group (n = 32), cecal ligation and puncture group (CLP group, n = 32) and Xuebijing treatment group (n = 32). The sepsis animal models in the last two groups were developed by CLP. In Xuebijing treatment group, animals were treated intravenously with Xuebijing injection (4 ml/kg) at 2, 12, 24,36, 48 and 60 hours after operation, respectively. Animals were sacrificed respectively at 6, 12, 24, and 72 hours after operation to harvest blood and hepatic tissue samples. Markers of liver function, contents of GSN and Gc globulin and expression of GSN mRNA in the liver tissues were detected. Results Compared with normal control group and sham operation group, hepatic GSN content was decreased significantly at various intervals in CLP group (P ＜ 0. 05), which reached the lowest value of (1.42 ±0.50) ng/mg at 12 hours. Meanwhile, expression of GSN mRNA in the liver was markedly enhanced at six hours after CLP and remained a high value until 72 hours, compared with the sham operation group (1.203±0.053 vs 1.030 ±0.084, P ＜0.05). Similarly, the hepatic Gc globulin contents was increased in the CLP group compared with the normal control group and sham operation group (P ＜ 0.05).After treatment with Xuebijing injection, the hepatic GSN contents at 6-72 hours was increased gradually while serum ALT and AST levels at 6, 12 and 24 hours were reduced significantly in comparison to the CLP group (P ＜ 0.05). Nevertheless, treatment with Xuebijing injection showed no marked effect on hepatic GSN mRNA expression and Gc globulin release (P ＞ 0.05). Conclusions Hepatic GSN contents are persistently reduced in the development of sepsis and treatment with Xuebijing injection can markedly attenuate acute liver injury through increasing GSN contents in severe sepsis.

Clinical significance of the changes of plasma homocysteine,endothelin,mononitrogen monoxide,folic acid and vitamine B_(12) in acute cerebral infarction / 中国基层医药

Objective To detect the fluctuations of homoeysteine(Hcy),endothelin(ET),mononitrogen monoxide(NO),folic acid(FA) and vitamine Bi2 (VitB_(12)) in the plasma of patients suffered from acute cerebral infarction and to investigate the clinical significance.Methods 140 blood specimens from cerebral infarction patients and 61 specimens from healthy individuals were obtained in this study and a series of items of Hey,ET,NO,FA and VitB_(12) were detected.Results The Hey [(17.15±4.63) μmol/L]and ET [(32.62±18.76) μmol/L]were increased in acute cerebral infarction(ACI) group compared with that in control group[(10.12±2.62) μmol/L,(20.08±13.38) μmol/L respectively]and the difference was significant (P＜0.01).The levels of NO [(39.82±7.26) μmol/L],FA [(7.54±4.01) ng/ml]and VitB_(12) [(247.70±119.85) pg/ml]were decreased compared with control group [(60.22±6.28) μmol/L,(10.08±3.86) ng/ml,(411.52±194.58) pg/ml respectively]and the differences significant (P＜0.01).Gender and hypertension affected the values of Hey.Conclusions Hcy and ET were increased while NO,FA and VitB_(12) were decreased in acute cerebral infarction.Hcy had positive correlation with ET and negative correlation with FA and VitB_(12).

The effects of calbindin-D28k on mice skeletal growth / 中国医师杂志

Objective To investigate the role of Catbindin-D28k in the kidney on calcium metabolism.Methods VDR/CaBP-D28k double knockout(KO)mice was made.Body weight,diet intake and serum,urinary parameters and length,density of the long bones,histological staining of the tibia of WT,CaBP-D28(-/-),VDR(-/-)and VDR(-/-)/CaBP-D28k(-/-)mice were determined on regular and high Ca-Lac diet.Results On a regular diet,the double KO mice were growth-retarded more and smaller than VDR KO mice.Compared with VDR KO mice,the double KO mice had higher urinary calcium excretion and rachitic skeletal phenotype,which were manifested with higher serum parathyroid hormone levels,lower bone mineral density,and more distorted growth plate with mole osteoid formation in the trabecular region.On high calcium and high lactose diet,blood-ionized calcium levels were normal in both VDR KO and the double KO mice.However,in contrast to VDR KO mice,the skeletal abnormalities were not completely corrected in the double KO mice.Conclusion These results directly demonstrate that CaBP-D28k plays a critical role in maintaining calcium homeostasis and skeletal mineralization and suggest that its caleemic role can be mostly compensated by CaBP-D9k.

Experimental study about the effect of Vitamin E and sodium selenite on nonalcoholic fatty liver / 重庆医科大学学报

Objective:To study the effect of Vitamin E(VitE)and Sodium selenite on nonalcoholic fatty liver(NAFL).Methods:24 SD rats were randomly divided into 3 groups.The model group,treatment group and contrast group were respectively fed with high fat diet,interfering diet and normal diet.All animals were sacrificed at the end of the 5th week.The liver pathology was observed under the light microscope.Superoxide dismutase(SOD),malondialdehyde(MDA)were determined by biochemistry analysis.The expressions of nuclear factor kappa B(NF-kB)and tumor necrosis factor alpha(TNF-?)proteins in hepatocytes were examined by immunohistochemistry.Result:①compared with the contrast group,serum and liver SOD levels decreased in model group,while MDA were raised.The expressions of NF-kB and TNF-a proteins in liver tissue increased significantly in model group.②compared with the model group,serum and liver SOD level increased in treatment group,while MDA was lowered.The expression of NF-kB proteins in liver tissue was reduced in treatment group,and no significant changes occured in TNF-a protein expression.Conclusions:Combination of sufficient quantum of VitE and Sodium selenite can improve the SOD activities and reduce the expression of NF-kB proteins in liver tissue,which is possibly the important mechanism for VitE and Sodium selenite to prevent NAFL.

Spinal and Supraspinal Changes in Vitamin D3 Up-regulated Protein 1 (VDUP1) Gene Expression after Spinal Cord Injury / 대한마취과학회지

BACKGROUND: Vitamin D3 up-regulated protein 1 (VDUP1) gene is known to be a novel member of the early response genes and an oxidative stress mediator. This study was designed to elucidate VDUP1 expression and its involvement in central sensitization after spinal cord injury (SCI). METHODS: Contusion injury was produced at spinal segment T10 (20 mm drop, 10 g rod) in adult male Sprague-Dawley rats (250 300 g). Withdrawal responses were measured using von Frey filaments and acetone on the 1st, 3rd and 7th days after SCI. The expressions of VDUP1 gene in the brain and in the cervical and lumbar spine were examined by immunohistochemistry on the 1st, 3rd and 7th day after SCI. RESULTS: VDUP1 gene was detected in a few oligodendrocytes in the spinal cord and in the brain of control rats. VDUP1 gene expression increased in most of the neurons and ependymal cells in the central canal of the injured (lumbar) spinal cord 1 day after SCI. This expression gradually decreased in majority of cells from day 1 to day 7 after SCI. VDUP1 gene expression was also observed to be increased 1 day after SCI, and gradually to decrease from 1 day to 7 days after SCI. The neurons in the intact (cervical) spinal cord VDUP1 gene expression increases maximally 3 days after SCI in the cerebral cortex and the thalamus. Neuropathic pain behavior was triggered by the plantar surface of the fore foot after SCI. CONCLUSIONS: These results show that the VDUP1 gene may be an early modulator of transneuronal stress response after SCI, and to be related to the central sensitization of neuropathic pain behavior after SCI.

Methylenetetrahydrofolate reductase polymorphism, homocysteine, lipid levels and susceptibility to preeclampsia in Korean women / 대한산부인과학회잡지

OBJECTIVE: The purpose was to test the hypothesis that the common missense mutation of 5,10-methylenetetrahydrofolate reductase(MTHFR) is more prevalent among preeclamptic women compared with control and also was to determine whether homocysteine and other lipid profile is changed in pregnant women with preeclampsia. METHODS: We measured plasma homocysteine, cholesterol, HDL, LDL, triglyceride, apoprotein B, vitamin B12, and folate in 48 pregnant women without preeclampsia and 22 women with preeclampsia. And the MTHFR genotype was determined with a polymerase chain reaction/restriction fragment length polymorphism method. Results were analyzed with a X2 contingency table and T-test. RESULTS: The prevalence of the MTHFR C677T mutation was not significantly different between the population studied. There was no significant difference in the level of plasma homocysteine, cholesterol, HDL, LDL, triglyceride, apoprotein B, and folate between controls and preeclamptic women. Furthermore, there was a significant difference in the level of plasma vitamin B12 between the population studied. CONCLUSION: These data suggest that the MTHFR C677T mutation is not a risk factor for preeclampsia in this population. Plasma homocysteine, cholesterol, HDL, LDL, triglyceride, apoprotein B, and folate level are not elevated in preeclamptic women. However, the plasma vitamin B12 level is elevated in preeclamptic women. Further studies are necessary to determine why the plasma vitamin B12 level is elevated in preelamptic women although they did not have vitamin drug.

Methylenetetrahydrofolate reductase polymorphism, homocysteine, lipid levels and susceptibility to preeclampsia in Korean women / 대한산부인과학회잡지

OBJECTIVE: The purpose was to test the hypothesis that the common missense mutation of 5,10-methylenetetrahydrofolate reductase(MTHFR) is more prevalent among preeclamptic women compared with control and also was to determine whether homocysteine and other lipid profile is changed in pregnant women with preeclampsia. METHODS: We measured plasma homocysteine, cholesterol, HDL, LDL, triglyceride, apoprotein B, vitamin B12, and folate in 48 pregnant women without preeclampsia and 22 women with preeclampsia. And the MTHFR genotype was determined with a polymerase chain reaction/restriction fragment length polymorphism method. Results were analyzed with a X2 contingency table and T-test. RESULTS: The prevalence of the MTHFR C677T mutation was not significantly different between the population studied. There was no significant difference in the level of plasma homocysteine, cholesterol, HDL, LDL, triglyceride, apoprotein B, and folate between controls and preeclamptic women. Furthermore, there was a significant difference in the level of plasma vitamin B12 between the population studied. CONCLUSION: These data suggest that the MTHFR C677T mutation is not a risk factor for preeclampsia in this population. Plasma homocysteine, cholesterol, HDL, LDL, triglyceride, apoprotein B, and folate level are not elevated in preeclamptic women. However, the plasma vitamin B12 level is elevated in preeclamptic women. Further studies are necessary to determine why the plasma vitamin B12 level is elevated in preelamptic women although they did not have vitamin drug.

Influence of plasma homocystine level on the prognosis of acute cerebral infarction and the effect of interventional therapy / 临床神经病学杂志

Objective To explore the influence of plasma homocystine(Hcy) level on the prognosis of acute cerebral infarction(ACI) and the effect of interventional therapy.Methods The plasma Hcy levels of 152 patients with ACI were measured by fluorometric method at

Growth inhibition of human breast cancer by vitamin E succinate combined with chemotherapeutic drugs / 中国普通外科杂志

Objective To investigate the inhibitory effect of vitamin E succinate(VES) combined with ~chemotherapeutic drugs on the proliferation of human breast cancer cells. Methods Bcap-37 human breast cancer cells were treated with VES combined with chemotherapeutic drugs for 24h and 36h. The ~concentrations of VES were 10?g/mL and 20?g/mL and those of 5-florouracil, mitomycin and ~cyclophosphamide were 16.9?g/mL and 33.8?g/mL, 1?g/mL and 3.3?g/mL and 100?g/mL and 300?g/mL respectively. The inhibitory effect was measured with MTT method and the cell cycle and cell ~surface Fas expression were analyzed with flow cytometry assay. Results The combination of VES with ~chemotherapeutic drugs had a significant inhibitory effect on the growth of Bcap-37 human breast cancer cells. Flow cytometry assay of cell cycle showed that the natural apoptptic rate of Bcap-37 cells was 0.7%;after treatment with VES 20?g/mL,the apoptotic rate was 19.2%;after treatment with 5-Fu,mitomycin and ~cyclophosphamide the apoptotic rates were 16.2%,16.7% and 12.3%,respectively;after the combined use of VES and the 3 chemotherapeutic drugs,the apoptotic rates were 40.3%,44.8%,39.6%,~respectively .Fas expression in cancer cells increased after the co-administration of VES and chemotherapy drugs. Conclusions VES combined with chemotherapeutic drugs had significant inhibitory effect on the growth of Bcap-37 human breast cancer cells. The mechanism may be related to Fas upregulation on the surface of cancer cells.

Intracranial Hemorrhage in Infants Secondary to Vitamin K Deficiency

We had reviewed the cases 8 thirteen patients with intracranial hemorrhage secondary to vitamine K deficiency, admitted between March 1990 at February 1994. The results were as follows: 1) The most common age of onset was under 2 months(12 cases, 92.3%). Sex ratio between male and female was 3.3:1(10:3). Premature was one of all cases. 2) In feeding history, breast feeding(7 cases, 53.8%) was most common, followed by mixed feeding(4 cases, 30.8%) and formular feeding(2 cases, 15.4%). Breast feeding, with or without formula, constituted 11 cases (84.6%) and intracranial hemorrhage was developed in breast feeded babies often more than in formular feeded ones. 3) Clinical findings were variable: mental change(11 cases, 84%) was the most common feature, followed by irritability, poor sucking, vomiting, motor weakness and poor crying. 4) Subdural hemorrhage(9 cases, 69.2%) was most common and followed by intracerebral hemorrhage(7 cases, 53.9%) and intraventricular hemorrhage(3 cases, 23.1%), with or without other hemorrahge. 5) Prolongation of PT and vitamine K dependent coagulation factor deficiencies were noted in all cases. APTT was prolonged in 11 cases(84.6%), but it returned to normal in all cases after vitamine K administration. Vitamine k1 level checked in 5 cases was lower than normal. 6) Treatment included vitamine K administration, subdural tap, burr hole drainage, EVD or craniotomy. Short term result was not good(grade IV+V;8 cases(61.8%)), but long term outcome would be worse than short term outcome(normal ; 1 case). It is our believe that while not very common in Western nations.

Clinical and In Vitro Study of Viaminate in Acne / 中华皮肤科杂志

Objective To investigate the therapeutic effect of oral viaminate(a vitamine A acid derivative)on acne and its effect on the expression of androgen receptor.Methods Two hundred and eigh-teen patients with acne were allocated to treatment group and control group at random:treatment group was treated by oral viaminate;control group was treated by oral minocycline.The treatment lasted for four weeks.The effect of viaminate on the expression of androgen receptor was studied in COLO-16cells in vitro by using Northern blot or Western blot.Results The therapeutic effect of oral viaminate in treatment group was higher than that in control group(P

Treatment of herpes simplex keratitis with combination of poly Ⅰ: C, Vitamine C and a small dose of dexamethasone / 眼科

44 eyes (42 patients) of HSV keratitis were treated by a combined injection of poly Ⅰ: C, vitamine C and a small dose of dexamethasone with satisfactory results. 22 (88％) of 25 eyes of the superficial type were cured and 2 eyes(8％) improved; 13 (68.4％) of 19 eyes of the deep stromal type were cured and 3 eyes (15.8％) improved. The average therapeutic course was 13.6 days for the superficial type and 25.4 days for the deep stromal type. 4 eyes (19.1％) recurred during a follow-up of 6-22 months. The mechanism of curative effects and the significance of the small does of dexamethasone were discussed.